Continue reading "paper chromatography report"
First of all pieces of Chromatography paper was taken and aline was drawn 2cm above the bottom of the page.
lhschem351: Paper Chromatography Lab
It is also used in the separation of molecules in a compound
If we use water as a solvent, then it will separate the colors in the mixture more effectively because it has the greater molecular attraction and intermolecular forces.
Conclusion (hopefully we are at 8 minutes by now)
In this lab we learned how to separate molecules from a mixture using chromatography.
This was done by separating the photosynthetic pigments (chlorophyll a, chlorophyll b, carotene and xanthophylls) from one another using paper chromatography.
8 Comments on "Chromatography Lab Answers"
Green plant have six closely related photosynthesis pigments(in order of increasing polarity): Carotene (orange pigment),XanthophyII (yellow pigment), Phaeophytin
(yellow-brown pigment), ChlorophyII
(blue-green pigment) and ChorophyII
The retention factor (R
may be defined as the ratio of the distance traveled by the substance to the distance traveled by the solvent
3.1 MATERIAL AND APPARATUS
3.2 ARRANGEMENT OF APPARATUS
How to identify the presence of difference pigments in chlorophyll's
What are the factors affecting these R
USING PAPER CHROMATOGRAPHY
80% of Acetone
"Cekur Manis" or Red Spinach Leaves
Mortar and Pestle
Muslin (cheese cloth)
Boiling Tube/Large Test Tube With Stopper (24x150mm)
Solvent ( 1 Part 80% Acetone and 9 Part Petroleum Ether)
Whatman no.1 Chromatography Paper Strip (20 X 130mm)
Dark Paper/Black Sugar Paper
Retort Sand/ Test Tube Rack
Diagram 1: Assembling of chromatography apparatus
Liquid (paper) chromatography:
It generally is oriented towards the general detection and potential identification of chemicals in the presence of other chemicals in a complex mixture.
PIGMENT SEPARATION USING PAPER CHROMATOGRAPHY …
l Large test (24 * 150 mm);
l Stopper to fit test tube;
l A small glass tube to transfer pigment solution;
l Chromatography paper or filter paper;
l Rack of test tube;
l Pigment solution;
l Solvent (5 cm3).
Last but not least to record all the data in a table.
4.0 DATA INTERPRETATION AND DICUSSION
Distance moved by the pigment (mm)
Distance moved by the solvent (mm)
4.1 RESULT/TABULATION OF DATA
From the experiment,the pigments that exist in the plant based on their colors were chlorophyll a which show green color, chlorophyll b which was blue green,xanthophyII which was grey and carotene which was orange in color.
the factors that affecting the movement of pigment during chromatography were porosity of the chromatography paper,solubility of the solvent as well as the molecular size of the solute.
the pigment which moved the furthest was carotene by 102 nm in distance, this indicates that the soluble pigment carotene dissolved into the acetone easiest and thus moved furthest.
it is very soluble in the solvent being used and because it forms no hydrogen bond with cellulose.
beside the large value of R
value also indicates that carotene was smallest in size.
The molecular size of the pigment is inversely proportional to the R
To obtain a clear and accurate chromatogram the students were advised to use gloves.
PIGMENT SEPARATION USING PAPER CHROMATOGRAPHY ..
separation of pigments of photosynthesis using paper chromatography
(paper) chromatography: ..
Paper Chromatography Lab Report | Chromatography | …
Paper chromatography analyzes mixtures by separating the chemical contents onto paper
Hypothesis If I am to put a chromatography paper into a ..
Hypothesis For Plant Pigment Chromatography Lab
Chromatography lab hypothesis - One Turning Leaf
I would recommend not
taking out the pigment until the last minute when it is needed in
order to minimise the effect of light;
l An alternative technique (thin layer chromatography) mentioned in
the preparation paper can be employed: instead of absorptive paper, a
glass of plastic slide (or aluminium sheet) coated with silica gel is
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