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Synthesis of peptide bonds by proteinases.

M., Synthesis of bio-inspired hybrid polymers using peptide synthesis and protein engineering.

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Accelerated chemical synthesis of peptides and small proteins.

Then, the enzymatic synthesis of peptides with proteolytic enzymes is reviewed considering medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed.

This strategy is used for the synthesis of small to medium size peptides having up to 50 residues.
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There are several examples of proteases and other peptide bond forming enzymes which have been successfully employed in peptide synthesis in such media (

Custom Peptide Synthesis Services($1.9/AA) - …

Chemical protein synthesis by solid phase ligation of unprotected peptide segments.
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Useful methods in enzymatic synthesis of peptides: a comparative study focusing on kinetically controlled synthesis of Ac-Phe-Leu-NH2 catalyzed by α-chymotrypsin.

It is important to point out that the use of organic solvents usually required for performing enzymatic peptide synthesis imposes additional restrictions on process design and engineering (

Buy Peptide Online | Peptide Online Quote - Selleckchem

Protease-catalyzed synthesis of peptides in biphasic aqueous-organic systems.
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This is a complex task in the case of the synthesis of peptides because of the complexity and the number of operations involved in the production process.

This is particularly so in the case of the chemical synthesis of peptides, which involve coupling, protection and deprotection reagents that are environmentally aggressive and even toxic (

Immunization of cattle with synthetic peptides derived from the  gut protein (Bm86).
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  • GENEPEP - PEPTIDES, PEPTIDE LIBRARIES AND PROTEINS

    S.; Wong, C.-H., 1,2,3-triazole as a peptide surrogate in the rapid synthesis of HIV-1 protease inhibitors.

  • Glossary | Linus Pauling Institute | Oregon State University

    Chemical ligation is a particularly appealing strategy for the chemical synthesis of large peptides and proteins (

  • Protease Inhibitor Technical Information

    Subtilisin, chymotrypsin, trypsin and papain have been widely used proteases in the chemical synthesis of peptides.

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Peptide Hormones - The Medical Biochemistry Page

The fragments that contain the whole peptide or protein sequence are condensed in solution to yield a product with protected side chains which is then deprotected and purified to complete the synthesis of the desired product.

The Ulijn Group - Nanoscience at ASRC/CUNY

The proteases that are used for peptide synthesis are selected on the basis of their specificity against amino acid residues on either side of the splitting point () and include the majority of the commercially available endo and exoproteases.

Prof Rein Ulijn PhD Msc FRSC FRSE

Commonly, peptides of less than 30 residues are produced entirely by solid phase sequential synthesis (), while larger peptides (up to 60 residues) must be produced by convergent synthesis in which protected peptide fragments are synthesized by SPPS and then combined by liquid phase synthesis (

Rein Ulijn's research is focused on peptide nanotechnology

The broad specificity of proteases restricts their application in peptide synthesis, since the peptide product that accumulates during the reaction can be attacked by the proteases simultaneously with the reaction of synthesis (

Click Chemistry -- Publications

However, the replacement of piperidine by stronger bases has circumvented the problem of incomplete deprotection in Fmoc/tBu solid phase synthesis of β-peptides () and longer β-peptides and mixed type α-β-peptides have been successfully synthesized by chemical ligation (

Nucleotide Metabolism: Nucleic Acid Synthesis

Small β-peptides can be synthesized using conventional Fmoc/tBu SPPS strategy (), but the protocols have to be modified for longer peptides because of the high folding propensity of β-peptides, which increases coupling times and prevents complete deprotection.

Protease Inhibitors - Enzyme Inhibitors | Sigma-Aldrich

On the other hand, non-proteolytic enzymes can also be used in connection with peptides, as illustrated by the kinetic resolution of N-phenylacetyl-DL-tert-leucine in the production of the non-proteinogenic chiral amino acid L-tert-leucine recently reported with penicillin G acylase from (

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